Staining of Toxoplasma in Histological Sections

Staining of toxoplasma in histological sections.

EXTRACELLULAR toxoplasma, although present in abundance in smears from animals infected experimentally, can only occasionally be found in sections from suspected toxoplasma lesions. In the eye, Wilder (1952a, b) has described organisms morphologically indistinguishable from toxoplasma in cases of granulomatous uveitis, but even in these sections the organisms were not numerous and were found on...

A comparison of methods for staining tubercle bacilli in histological sections.

The Ziehl-Neelsen stain is compared with three alternative methods of staining tubercle bacilli in paraffin sections: Fite's method (1938); a modification by Armstrong and Price (1947) of Fite's method; and a fluorescent method using the auramine-phenol stain. The fluorescent method was found to be the most effective of the four methods and its use is recommended as a routine tool in diagnostic...

Histological staining techniques

The first stainings were tried by A VAN LEEUWENHOEK (apparently in the years 1714-1719) and by R VIEUSSENS (about 1684) studying their objects with alcoholic saffron dyes (LEWIS FT, 1942). At last, genuine histological work began much later when T HARTIG, C WEIGERT, J GERLACH, P EHRLICH and H GIERKE started in the second half of the 19 century with their systematic studies of natural and synthe...

Comparison of Three Staining Methods of Vibratome and Paraffin Sections in Preserving Thickness of Rat Hippocampal Sections

Purpose: To determine the optimal technique for maintaining hippocampus vibratome sections volume. Materials and Methods: This experimental study was performed on three rat hippocampus. The first hippocampus was embedded in paraffin and using rotary microtome forty sections were cut in a nominal thickness of 100 micrometer. They were then ordinary stained. The two others were cut into 80 sectio...

Non-nuclear staining of thick tissue sections.